The range this line is obviously meant for is a villain with 66-99 Tx, JJ. Given your read on the player, I don't think the river bet is unreasonable if you don't think he plays Qx+ this way, and will fold less. There are a lot of hands ahead of you which are also giving you fold equity.

The answer is yes, against a very specific read. You just have to know that good players will check Qx+ on this river a lot with the intention of pot control and a look up, snap off scenerio when you bet.